APP-Beta-CTF ELISA 27776 from IBL- America

Product Specs
ItemAPP-Beta-CTF ELISA
CompanyIBL- America
Price Pricing Info Supplier Page View Company Product Page
Catalog Number27776
Quantity96 Determinations
ApplicationsELISA Kit
Regulatory StatusFor research use only, not for use in diagnostic procedures
Detection Range0 / 0.19 - 12 pmol/L
ReactivityHuman
Sample TypeCell lysate
Sensitivity0.02 pmol/L
Volume100 µL of properly diluted unknown / determination
Assay TimeOvernight incubation (4°C) + 1 hour (4°C) + 30 min. (RT) = Overnight + 1 hour, 30 min. total incubation time
Storage2 - 8 °C

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IBL- America

8201 Central Ave NE Suite P

Minneapolis, Minnesota 55432

United States

Phone: 888-523-1246
763-780-2988

Company Profile

Website: http://www.ibl-america.com/

(1) APP-BACE1 Interaction and Intracellular Localization Regulate Aβ Production in iPSC-Derived Cortical NeuronsCellular and Molecular NeurobiologyOctober 1, 2023Sandra Roselli, Tugce Munise Satir, Rafael Camacho, Stefanie Fruhwürth, Petra Bergström, Henrik Zetterberg, Lotta AgholmeScale Discovery). Intracellular CTFβ was measured with a human APP βCTF Assay Kit (IBL, catalog number 27776) as described by the manufacturer. Briefly, cells were lysed in MSD lysis buffer (150 mM NaCl, 20 mM

(2) Trem2 H157Y increases soluble TREM2 production and reduces amyloid pathologyMolecular NeurodegenerationJanuary 31, 2023Wenhui Qiao, Yixing Chen, Jun Zhong, Benjamin J. Madden, Cristine M. Charlesworth, Yuka A. Martens, Chia-Chen Liu, Joshua Knight, Tadafumi C. Ikezu, Aishe Kurti, et al.commercial kits (Meso Scale Discovery, K15120E-2). CTFβ in TBSX lysates was detected by commercial kit (IBL, 27776). TNFα was measured in TBS lysates using commercial kit (Meso Scale Discovery, K152QWD-1). TREM2 in

(3) Concomitant Retinal Alterations in Neuronal Activity and TNFα Pathway Are Detectable during the Pre-Symptomatic Stage in a Mouse Model of Alzheimer’s DiseaseCellsMay 16, 2022Virginie Dinet, Louiza Arouche-Delaperche, Julie Dégardin, Marie-Christine Naud, Serge Picaud, Slavica Kranticprotein extracts, as per procedure used for the western blot. A commercially available ELISA kit (Cat n°. 27776, IBL International GmbH, Tecan, Männedorf, Switzerland) was used for βCTF (sensitivity 0.02 pmol/L)

(4) APOE4 exacerbates synapse loss and neurodegeneration in Alzheimer's disease patient iPSC-derived cerebral organoidsNature communicationsNovember 2, 2020Zhao J, Fu Y, Yamazaki Y, Ren Y, Davis MD, Liu CC, Lu W, Wang X, Chen K, Cherukuri Y, et al.Human sAPPα Assay Kit (IBL, 27734), Human sAPPβ Assay Kit (IBL, 27732), Human CTF-β ELISA kit (IBL, 27776), and the Tau (Phospho) [pS396] Human ELISA Kit (Thermo Fisher, KHB7031) according to the manufacturers’

(5) A cellular complex of BACE1 and γ-secretase sequentially generates Aβ from its full-length precursorThe Journal of cell biologyFebruary 4, 2019Liu L, Ding L, Rovere M, Wolfe MS, Selkoe DJ.specific), 18584 (rabbit, RRID: AB_2341377; IBL). For the commercial ELISA assay, ELISA for APP-CTFβ, 27776 (IBL); for sAPPβ-sw, K151BUE (Meso Scale Discovery); and for BACE1, DY931 (R&D Systems) were used, and

(6) CRISPR Transcriptional Activation Analysis Unmasks an Occult γ-Secretase Processivity Defect in Familial Alzheimer's Disease Skin FibroblastsCell reportsNovember 14, 2017Inoue K, Oliveira LMA, Abeliovich A.(#KHB0051, Thermo Fischer Scientific). APP-βCTF levels were measured using a human APP-βCTF Assay kit (#27776, Immuno-Biological Laboratories). BACE1 levels were measured using a BACE1 Assay kit (#27752, Immuno-Biological Laboratories

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Bench Tips for Optimizing ELISA: It All Comes Out in the Wash
August 22, 2013
This article will provide you with tips on washing your ELISA plates using an automated plate washer, with the goal of avoiding high background and high variation (between plates and between replicate wells). read more

APP-Beta-CTF ELISA from IBL- America

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IBL- America

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Bench Tips for Optimizing ELISA: It All Comes Out in the Wash

APP-Beta-CTF ELISA from IBL- America

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